|     home    |     search     |     content     |     mail     |     login     |

study information

date of publication30-jul-1999
journalJ Immunol Methods 1999 Jul 30;227(1-2):85-97
titleStandardisation and quality assurance of lymphocyte proliferation assays for use in the assessment of immune function. European Concerted Action on Immunological and Virological Markers of HIV Disease Progression.
title in Dutch
resp. centreCLB
designLymphocyte proliferation is a widely used technique to assess immune competence. However, the technique is subject to a large degree of variation, some biological and some technical. In this study, the components of variation in whole blood proliferation assays were analysed over time, using both antibody and mitogenic stimulants. The levels of variation within individual samples, between individuals and between groups of individuals over time were examined. A method of transforming the data is proposed which reduces the coefficients of variation to an acceptable level, and which expresses individual results as a standardised count. This method overcomes the problem of different levels of absolute counts, it corrects for time sensitive errors and allows data from multiple laboratories to be pooled.

Use this form to to view general information about a study.
  • click on 'authors' to view the list of authors who contributed
  • click on 'files' to view a list of files, associated with this study: these files can be of any type: f.i. the full text of the article, or code used in the analysis, or a Powerpoint demonstration.
  • click on 'tables' to view a list of the database tables that were used in this study: more is information on these tables is available.
  • for studies related to the Amsterdam Cohort, the option 'progress' is available: it gives information about the process of collecting information about a study: who was asked for information, when was this done, is the information complete, etc.
  • if you have the authority to change data about the study, the option 'edit' is available: clicking this will take you to a form for editing the main information, with options to edit all aspects.
  • clicking on the PubMedID opens a new browser window with PubMed
  • clicking on the 'resp. centre' gives a list of all studies for this centre
  • click the 'back' button to go to the previous document